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KMID : 0363620100310060008
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Journal of Korean Oriental Medicine
2010 Volume.31 No. 6 p.8 ~ p.15
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Simultaneous Determination of Albiflorin, Cinnamaldehyde, Cinnamic Acid, Daidzin, Glycyrrhizin, Liquiritin, Paeoniflorin and Puerarin in Galgeun-tang by HPLC-PDA
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Seo Chang-Seob
Kim Jung-Hoon
Shin Hyeun-Kyoo
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Abstract
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Objectives: We investigated to develop and validate HPLC-PDA methods for simultaneous determination of eight constituents in Galgeun-tang (GGT).
Methods: Reverse-phase chromatography using a Gemini C18 column operating at , and photodiode array (PDA) detection at 230 nm, 254 nm, and 280 nm, were used for quantification of the eight marker components of GGT. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid.
Results: Calibration curves were acquired with > 0.9999, and the relative standard deviation (RSD) values (%) for intra- and inter-day precision were less than 3.0%. The recovery rate of each component was in the range of 87.33-101.38%, with an RSD less than 7.0%. The contents of the eight components in GGT were 1.98-12.17 mg/g.
Conclusions: The established method will be applied for the quantification of marker components in GGT.
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KEYWORD
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HPLC-PAD, Galgeun-tang (GGT), simultaneous determination, herbal formula
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